NJBMS - Volume 1, Issue 3, January - March 2011

Pages: 133-136


Author: Satish Kumar NS , Aswini Dutt R , Maruthy KN , Dinakar Nadig , Neevan D R Dsouza

Category: Physiology

[Download PDF]


The red cell sizes are described in the scale of micrometers. For a novice it is difficult to imagine the cell size. Measuring cell size requires the use of advanced instruments whichmandates use of trained personnel. To measure the red cell size using a custom built microscope camera and computer based image analyzing software. Sixty peripheral blood smearswere photographed using a microscope camera. The images were stored in a computer. The retrieved images were measured offline using the software. Two independent observers recorded the red cell sizes on both randomly selected and tagged red cells of each slide. Themeanredcell widthof thetaggedredcells as measured by the first and second observer was 7.70-7.82 (}0.61μ) and 7.71-7.76 (}0.61μ) respectively. The measured red cell width ranges between 7.49-7.67 (}0.54μ) and 7.34-7.44 (}0.60μ) in untagged red cells for both the observers. The intraclass correlation coefficient for inter rater &I ntra rater reliability is 0.956 (observer 1) and 0.944 (observer 2) for tagged red cell width and 0.723 (observer 1) and 0.730 (observer2) foruntaggedredcell width. Computer based image analysismethod to determine red cell size, provides an accurate and reliable measurement, which is simple and cost effective. This method can be used to demonstrate measurement of red cell width to life-sciences students in the setting of hematology practical.

Keywords: Red cell width, Image analysis, Microscopicmeasurement, Red blood cell.

No. of online users: 100

Search Articles

Visitor Counter

World wide visitors


Editor in Chief of NJBMS Dr.Deepti Shastri, Professor and Head, Department of Anatomy, Academic Co-ordinator for Pre and Paraclinical Sciences, VMKV Medical College & Hospital, Salem.

Indexed in

Index Copernicus (MCI recognized indexing body) ICV - 75.97, Scientific Indexing Services, Research Bib, Indian Science Publication, Eurasian Scientific Journal Index, Science Library Index, International Innovative Journal Impact Factor, Cite Factor, International Impact Factor Service,

Publication Statistics

Articles Statistics


Society for Basic Medical Sciences
NJBMS Journal - Indexed in Index Copernicus

Manuscript Statistics

Subscribe for TOC Alerts

Get the table of contents of current issues.

E-mail Alerts