UA-22728437-71


NJBMS - Volume 5, Issue 1, July - September 2014

Pages: 13-16

COMPARISON OF SUSCEPTIBILITY TESTING METHODS IN DETERMINING VANCOMYCIN MIC IN METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES

Author: J. MOWNA, K.M. MOHANA SOUNDARAM, R. INDRA PRIYADHARSINI

Category: Microbiology

[Download PDF]

Abstract:

Background: In 1980 due to widespread occurrence of methicillin-resistant Staphylococcus aureus(MRSA), empiric treatment was changed to Vancomycin in many health care institutions. According to studies, Vancomycin minimum inhibitory concentration (MIC) by E-test was 1-2-fold dilutions higher than the MIC by agar dilution and broth dilution. This study was undertaken to find out the susceptibility pattern of vancomycin and to compare agar dilution and E test.Aim: The aim was to compare the susceptibility testing methods in determining vancomycin MIC in MRSA isolates.

Methodology: This prospective study was conducted between November 2011 to May 2013 in the Department of Microbiology Vinayaka Missions Kirupananda Variyar Medical College,Salem.A total of 218 MRSA isolated from heterogenous clinical samples received in Microbiology laboratory were stored in nutrient agar vials at 4-10 oC till further processing. Vancomycin MIC was determined by agar dilution, and E -test method, and MIC creep (2µg/ml) was determined.

Results: In the present study, of the 218 isolates, 185 were VSSA (MIC 0.5 - 2 µg/ml), 33 isolates were VISA (MIC - 4-8 µg/ml) and no vancomycin resistant S.aureus isolated (MIC >16 µg/ml). The MIC creep (2µg/ml) was 11 %. In the present study, the E test MIC was higher than the agar dilution method.

Conclusion: Staphylococci with reduced susceptibility to vancomycin was observed in the present study.Hence, as a precautionary measure before starting a patient on vancomycin, the clinicians should seek the help of clinical Microbiologist to determine the MIC of such strains sand hence that emergence of vancomycin resistance can be prevented.

Keywords: Agar dilution, E test, Minimum inhibitory concentration creep, Vancomycin minimum inhibitory concentration.

No. of online users: 82

Search Articles



Visitor Counter

World wide visitors

News

Editor in Chief of NJBMS Dr.Deepti Shastri, Professor and Head, Department of Anatomy, Academic Co-ordinator for Pre and Paraclinical Sciences, VMKV Medical College & Hospital, Salem.


Indexed in

Index Copernicus (MCI recognized indexing body) ICV - 75.97, Scientific Indexing Services, Research Bib, Indian Science Publication, Eurasian Scientific Journal Index, Science Library Index, International Innovative Journal Impact Factor, Cite Factor, International Impact Factor Service,

Publication Statistics

Articles Statistics

Advertisements

Society for Basic Medical Sciences
NJBMS Journal - Indexed in Index Copernicus

Manuscript Statistics

Subscribe for TOC Alerts

Get the table of contents of current issues.

E-mail Alerts