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NJBMS - Volume 3, Issue 4, April - June 2013

Pages: 281-288
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EVALUATION OF THE PROTECTIVE ROLE OF AQUEOUS EXTRACT OF MORINGA OLEIFERA LEAF AGAINST OXIDATIVE STRESS AND HISTOLOGICAL ALTERATIONS IN TESTES OF RATS UNDER INSULIN RESISTANT CONDITION

Author: DIVI. SAI M

Category: Biochemistry

Abstract:

Objective: To investigate the effect of aqueous extract of Moringa oleifera (AEMO) leaf on the oxidative stress, antioxidant status as well as histo pathological changes in testes of rats under insulin resistant condition.

Methods: The male Wistar rats were randomly divided into four groups of six rats each : C (Control), C+MO (control rats administered with AEMO), F (fructose fed rats) and F+MO (fructose fed rats administered with AEMO), Insulin resistance was induced by high fructose diet. AEMO was administered orally at a dose of 200 mg/kg body weight for 60 days. Relative organ weight was calculated. Phytochemical screening of AEMO was done by standard methods. Oxidative stress and the status of antioxidant enzymes were estimated in testes and the histological changes in testes were studied. Statistical significance of the results was evaluated by Duncan's Multiple Range Test.

Results: Phytochemical analysis showed the presence of pharmacologically important phytochemicals. The reduced relative weight of the testes was observed in F group. F group showed increased lipid peroxidation, decreased levels of reduced glutathione, reduced activities of glutathione reductase, glutathione S transferase, superoxide dismutase, catalase increased activity of Sorbitolcdehydrogenase. Histological pictures of testes of F group showed pathological changes. However, administration of AEMO corrected the alterations towards normalcy

Conclusion: In this study, AEMO ameliorated oxidative stress, exhibited antioxidant potential and played a role in the protection of testicular structural integrity under insulin resistant condition.

Keywords: Moringa oleifera, Insulin resistance, Testes, antioxidants, oxidative stress, Histopathology