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NJBMS - Volume 7, Issue 4, April - June 2017

Pages: 226-234
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Microbiological Study of Neonatal Sepsis Using Automated Blood Culture System at a Tertiary Health Care Centre

Author: Vaibhav V. Rajhans, Sharmila S. Raut, Sanjay R. More, Vimal S. Rathod, Vivek M. Gujar5

Category: Microbiology


Introduction: Sepsis in neonates is responsible for highly considerable mortality, especially in developing countries like India, where it is contributing 19% of neonatal deaths. Present study was undertaken to study aerobic bacterial profile of neonatal septicemia by automated blood culture systems, using BacT/ALERT 3D 60 blood culture system and miniAPI bacterial identification and antibiotic susceptibility testing system, considering the importance of urgent and accurate identification of the pathogen and to reduce the unnecessary therapeutic load on patients as well as clinicians.

Aim: To isolate and identify bacterial pathogens in neonatal sepsis and to know their antibiotic susceptibility pattern using BacT/ALERT and miniAPI systems.

Material and Methods: 157 blood samples from neonates suspected to be in sepsis were studied during the study period using BacT/ALERT 3D 60 systems for blood culture and miniAPI system for identification and antibiotic susceptibility of isolates.

Results: 70 blood cultures were flagged positive by BacT/ALERT. E.coli was the most common i.e 16 (22.85%); followed by Coagulase negative Staphylococcus (CoNS), isolated in 15 (21.42%), followed by Staphylococcus aureus isolated in 14 (20%).

Conclusion: Total blood culture positivity rate was 44.58%. E. coli was the most common pathogen isolated. By using BacT/ALERT 3D 60, 65.71% blood culture positivity was observed within 24 hours incubation and bottles flagged positive within 48 hours were 59 (84.28%). Both, the BacT/ALERT 3D 60 and the miniAPI systems, were efficient, less time consuming and labor saving which is essential for the clinician and more importantly for the patient.

Keywords: Neonatal sepsis, Bacterial sepsis, automated blood culture, BacT/ALERT, miniAPI