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NJBMS - Volume 9 Issue 2, October - December 2018

Pages: 71-78
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Detection of Coagulase negative Staphylococcal blood stream infections among Infants and Neonates and their Antibiotic susceptibility pattern in a Tertiary care Hospital

Author: Sowmya.A, Mathavi.S, Indra Priyadharsini.R

Category: Microbiology


Introduction: There has been an increased emergence of Coagulase negative Staphylococcus causing blood stream infections in Neonates and Infants leading to morbidity and mortality. Catheter related blood stream infections are most common in critically ill very low birth weight neonates and infants. Colonization and biofilm formation are the significant risk factors. Another concern is the rising incidence of Methicillin-resistant Coagulase negative Staphylococcus (MRCONS) in hospitalized childrens.

Aim & Objectives: This study was conducted to know the Prevalence of Coagulase negative Staphylococcus associated septicemia, to speciate them and to know the Antibiotic susceptibility pattern of these isolates.

Materials and Methods: The blood samples were collected from Neonates and Infants. Brain heart infusion (BHI) was used for blood culture. 1-2ml of blood was collected in 10ml of BHI broth. By repeated isolation, clinically significant Coagulase negative Staphylococcus was identified. From the positive isolates Gram staining, biochemical tests and Antibiotic susceptibility tests were done.

Results: Out of 362 blood samples, 92 (25%) shows clinically significant CONS. Biotyping shows

the commonest species isolated from blood samples were Staphylococcus epidermidis, Staphylococcus haemolyticus and Staphylococcus warneri. All the isolates were resistance to Penicillin. Many isolates were resistance to Methicillin (61%) and other commonly used drugs. All MRCONS isolates were 100% sensitive to Vancomycin.

Conclusion: The study demonstrates higher prevalence of CONS in neonatal and infant bacteremia. Staphylococcus epidermidis is the commonest pathogen among Coagulase negative Staphylococcus in our study.

Keywords: Coagulse negative Staphylococcus, Neonates and Infants septicemia, Staphylococcus epidermidis, Antibiotic susceptibility tests, Methicillin resistant Coagulase negative Staphylococcus.

DOI: 10.31975/NJBMS.2018.9202