Background : High density lipoprotein-cholesterol HDL-C is an independent risk factor for atherosclerotic cardiovascular disease. HDL-C levels can be modified by exercise training. However, the response of exercise training on HDL-C levels is highly variable. Such variability could be because of genetic variation of enzymes involved in HDL metabolism. Endothelial lipase(EL) is one such enzyme coded by LIPG gene . Objectives: The objective is to find out the association of LIPG 584 C > T gene polymorphism with baseline HDL-C and its sub-fraction levels. The objective is also to find out the response of exercise training on HDL-C and its sub-fraction levels in relation to LIPG 584C >T gene polymorphism.
Materials and Methods: Interventional Study 60 apparently healthy subjects of 30-65 years of age, newly enrolled in the fitness centre were selected. Baseline assesment of fasting lipid profile, HDL subfractionation, LIPG 584C > T gene polymorphism using TaqMan assay by real time Polimerase chain reaction was done. Subjects underwent 16 weeks of supervised aerobic exercise training. After 16 weeks, fasting lipid profile, HDL subfractionation were repeated.
Results : At baseline, statistically significant difference of HDL-C and its sub-fraction between three genotypes was noted by Analysis of Variance (p < 0.05). By paired t-test, CC genotype subjects showed statistically significant difference of total HDL-C and its sub-fraction (p < 0.05) but TT genotype subjects did not show statistically significant difference between total HDL-C and its sub-fraction before and after exercise training (p > 0.05).
Conclusion : EL gene variants were associated with alteration of HDL-C and its subfractions at baseline and after exercise training. Therefore EL inhibitors could be considered as potential target to raise HDL-C levels.
Keywords: Atherosclerosis, Endothelial lipase, Exercise training, High density lipoprotein \� cholesterol, LIPG gene, Single-nucleotide polymorphism.
