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NJBMS - Volume 1, Issue 4, April - June 2011

Pages: 209-211
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PREVALENCE OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS AMONG HEALTHCARE WORKERS

Author: RAO VENKATAKRISHNA I , BHAT KISHORE , KAVITHA , SHANTARAM MANJULA

Category: Microbiology

Abstract:

Problem statement:

Methicillin Resistant Staphylococcus aureus (MRSA) nosocomial infection outbreaks and prevalence among various populations are well reported in literature. Nosocomial infection due to MRSA is a known cause of increased hospital stay, morbidity and mortality especially in the critically ill patients. But there is paucity of information onMRSAin developing countries like India. There is no proper surveillance system and control policy forMRSA.

Methods:

One hundred and fifty healthcare workers were screened for MRSA carrier status and vancomycin susceptibility of the isolates was determined. Swabs of anterior nares were taken, cultured by using nutrient broth with 7.5% NaCl and Mannitol Salt Agar (MSA). Staphylococcus aureus was identified by conventional biochemical methods. MRSA detection was done by Modified Kirby-Bauer disc diffusion method by using oxacillin (1μg) and cefoxitin (30 μg) discs. Susceptibility to vancomycin was also determined by disc diffusion

method using vancomycin (30 μg) disc.

Result

Of the 150 healthcare workers screened, 18 had MRSA in the nose giving a carriage rate of 12%. MRSA isolated were resistant to commonly available antibiotics. Novancomycin resistancewas detected.

Conclusion

MRSA carriage among healthcare workers is high. The isolates were multi-drug resistant which may lead to increased morbidity and mortality if transmitted to the critically ill. There is need for MRSA control policies in the hospitals with normal settings.

Keywords: MRSA, Staphylococcus aureus, critically ill, healthcare workers.