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NJBMS - Volume 4, Issue 3, January - March 2014

Pages: 155-158
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CHARACTERISATION OF NONDIPHTHERIAL CORYNEBACTERIA ISOLATED FROM CLINICAL SAMPLES AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN

Author: S. MATHAVI, A.V. RAGHAVENDRA RAO, A. KAVITHA, G. SASIKALA, INDRA PRIYADHARSINI

Category: Microbiology

Abstract:

Introduction: Coryneform or the nondiphtherial Corynebacterium species remains a neglected group as contaminants. Theseorganisms havebeen associated with invasive disease, particularly in immunocompromised patients. Species like Corynebacterium amycolatum, Corynebacterium jeikeium , Corynebacterium minutissimum and Corynebacterium urealyticum are reported with increasing frequency. An alarming rate of antibiotic resistance is also documented among such organisms.

Aim and Objective: This study was done to find out the various species of clinically relevant Coryneforms and to determine their antibiogram.

Materials and Methods: A total of 857 clinical samples (Pus, wound swab, urine, blood, sputum and catheter tips) received in the Microbiology department during January 2013 to October 2013 were included in the study. They were subjected to Gram’s staining and culture on blood agar and McConkey agar. The Gram‑ positive bacilli (GPB) isolated were subjected to speciation based on the panel of reactions described by Von Graevenitz and Funke. The antibiogram was determined by the disc diffusion method on 5% sheep blood agar.

Results: Out of total 857 clinical samples, 42 (4.9%) samples showed pure growth of GPB. C. jeikeium was the predominant species isolated. Species like C. jeikeium and C. urealyticum were highly resistant to commonly used antibiotics.

Conclusion: Coryneforms can no longer be ignored as contaminants. Determining the antibiogram is necessary as species like C. jeikeium and C. urealyticum are highly resistant to commonly used antibiotics. In our study all these organisms are sensitive to vancomycin, linezolid and tigecycline. Hence these drugs can be considered in the empirical treatment against serious infections caused by these organisms.

Keywords: Corynebacterium jeikeium, Coryneforms, Tigecycline, Vancomycin