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NJBMS - Volume 4, Issue 2, October - December 2013

Pages: 114-119
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METALLOBETALACTAMASES PRODUCING CLINICL ISOLATES OF PSEUDOMONAS AND ACINETOBACTER SPECIES IN A TERTIARY CARE HOSPITAL

Author: SUMI GUPTA, PUSHPA DEVI, SITA MALHOTRA

Category: Microbiology

Abstract:

Introduction: Metallo-beta-lactamases (MBLS) mediated resistance is an emerging threat in clinical isolates of Pseudomonas spp. and Acinetobacter spp.. These strains are responsible for several nosocomial outbreaks in tertiary care centers across the world. It is well known that poor outcome occurs when patients with serious infections due to MBL producing organisms are treated with antibiotics to which the organism is completely resistant. Therefore, detection of these MBL producers is crucial for optimal treatment of critically ill patient's and to prevent the spread of resistance.

Material and methods: The present study was undertaken to determine the incidence of MBL production in 400 clinical isolates of Pseudomonas spp. and Acinetobacter spp. by IMP-Ethylene diamine tetraacetic acid (EDTA) disk synergy test and to study the antibiotic susceptibility pattern of MBL producers by Kirby Bauer disk diffusion method .

Observation: Of the 23 imipenem resistant isolates 17(73.91%) were found to be MBL producers. MBL producers were found to be 100% susceptible to colistin and polymyxin B. 47.06% to piperacillin/ tazobactam, 35.29% to amikacin, 23.53% to ciprofloxacin and 05.88% to gentamicin.

Conclusion: Our findings showed that there is a need to do surveillance to detect MBL producers, judiciously use carbapenems to prevent their spread and use effective antibiotics, such as piperacillin-tazobactum, amikacin, ciprofloxacin and gentamicin, after sensitivity testing for treatment.

Keywords: Pseudomonas, Acinetobacter, Metallo beta lactamase (MBL).