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NJBMS - Volume 4, Issue 2, October - December 2013

Pages: 120-122
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PRESUMPTIVE IDENTIFICATION OF CANDIDA SPECIES FROM VAGINAL SPECIMENS USING CHROMAGAR

Author: K.LAVANYA DEVI, G.SASIKALA, R.INDRA PRIYADHARSINI, K.R.RAJESH

Category: Microbiology

Abstract:

Background: Vulvovaginalcandidiasisiscausedby Candida albicans as well as non-albicans species of candida. The conventional methods of species identification by assimilation and fermentation are reported to be cumbersome. In order to facilitate rapid identification, several chromogenic substrate containing culture media have been developed which yield colonies with varying colours secondary to chromogenic substrates that react withenzymessecretedbymicroorganism.

Aim and objective : To evaluate the usefulness of CHROMagar over conventional methods for speciation of candida isolates.

Materials and methods : High vaginal swabs collected from 200 sexually active women [18- 65yrs] with symptoms who attended the outpatient gynaecology department in a tertiary care hospital from January 2012 to October 2012 were subjected to gram stain and culture. The isolated colonies were speciated using CHROMagar, germ tube test, sugar assimilation and fermentation tests. The species were identified by the colours produced by each isolate on CHROMagar in 24hours and by sugar assimilation and sugar fermentation tests in 48hours.

Results : A total of 71 candida isolates were obtained which included 26 Candida albicans, 23 Candida parapsilosis, 13 Candida glabrata, 5 Candida tropicalis and 4 Candida krusei in 24 hours on CHROMagar and in 48 hours by sugar assimilation and sugar fermentation tests. The sensitivity and specificity of CHROMagar candida for these 5 species was 100%.

Conclusion : The use of chromogenic medium is an easy, rapid and reliable method for the presumptive identification of candida species in 24 hours. CHROMagar is of great help for the clinical microbiologist to save time and cost for diagnosis.

Keywords: Vulvovaginal, candidiasisis