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NJBMS - Volume 4, Issue 1, July - September 2013

Pages: 37-41
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SCREENING FOR METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS AND DETECTION OF mecA GENE BY PCR AMONG THE HEALTH CARE WORKERS IN A TERTIARY CARE HOSPITAL

Author: DHEEPA N, RAJESH K R., SEETHA K S.

Category: Microbiology

Abstract:

AIMS & OBJECTIVES: The aim of the study was to determine the prevalence of MRSA and detection of mec gene among the Health Care Workers (HCWs). The objective of the study was to screen HCWs for Staphylococcus aureus carriage and detectionofmecAgene.

MATERIALS & METHODS : A total of 100 HCWs were screened for Staphylococcus aureus carriage. Using pre- moistened sterile cotton swabs, specimens collected from the anterior nares, palms and web spaces were cultured & identified by conventional methods for Staphylococcus aureus. The antimicrobial susceptibility test was performed. Staphylococcus aureus isolates were screened for methicillin resistance by Oxacillin ( 1 µg ) disc diffusion, Oxacillin screen agar ( 6 µg/ml) and Cefoxitin ( 30 µg ) disc diffusion& confirmedbydetectionofmecAgeneby PCR.

RESULTS : Out of the 100 HCWs, 23 % were found to be Staphylococcus aureus carriers. The overall MRSA carrier rate was 10 %. Out of 10 MRSA carriers, 7 were from nasal and 3 were from web spaces . All 10 MRSA were detected by Oxacillin screen agar and Cefoxitin disc diffusion as against 8 MRSA by Oxacillin disc diffusion method. Male HCWs harboured statistically significant MRSA. All 10 MRSA isolates were positive for mecA gene by PCR.

CONCLUSION : Our study concludes that MRSA was prevalent in 10 % of HCW's and anterior nares was the commonest site. Male HCWs were predominant carriers of MRSA.. Cefoxitin disc diffusion method can be used as an alternative to the technically demanding PCR.

Keywords: Methicillin Resistant Staphylococcus aureus(MRSA), Health Care Workers (HCW's).