Materials and Methods : fat extract of Withania somnifera(WS) was evaluated in various paradigms - elevated plus maze test (EPMT), light and dark box (L and DT) and open field test (OFT) to establish anxiolytic activity of the extract. In addition, motor coordination was also assessed by rota rod test (RRT). Diazepam 1 mg/kg served as a standard anxiolytic drug, administered orally.

Results: The fat extract of WS (100 and 200 mg/kg, p.o.) significantly increased the percentage of time spent and number of entries in open arm in EPMT. In L and DT, the extract produced significant increase in time spent, number of crossing and decrease in the duration of immobility in light box. In OFT, the extract showed significant increase in number of rearings and square crossed, all of which are demonstrations of exploratory behavior. Furthermore, the extract produced skeletal muscle relaxant effect assessed by RRT.

Conclusion: The results suggest that the extract has anxiolytic – like activity. It may be worthwhile isolating the constituents for further study