COMMERCIAL GRADE MALATHION INDUCED OXIDATIVE IMBALANCE IN MICE: THE ROLE OF ASCORBIC ACID
Author: D' SOUZA R. T. JANITA , BERNHARDT VIDYA , SHANTARAM MANJULA
Category: Microbiology
Abstract:
Studies on the ameliorating effects of ascorbic acid on the oxidative imbalance following exposure to commercial grade Malathion (CGM) preparations have not received much attention. The study was designed to understand the effects of ascorbic acid (250 mg/kg) on Swiss Albino mice (weighing 21 – 24 grams and in each group six animals), when three sublethal, subchronic doses of CGM,(50% LD ,25%LD , and5%LD )were administered orally for 14 days. At the end of 14 days the ameliorating effect was checked by estimating the levels of protein carbonyl (PC) in liver, superoxide dismutase (SOD) and catalase (CAT) in the RBCs. In this study, treatment with ascorbic acid showed significant amelioration of altered RBC CAT levels as compared to 5% LD CGM treatment alone. With the dosage of ascorbic acid selected in this study, attenuation of SOD and PC levels which were altered due to exposure to the three doses of CGM and CAT levels which were altered due to 50% and 25% LD CGM was not found to be significant statistically. The dosage of ascorbic acid must be standardized in order to obtain optimum response to satisfy the oxidative changes.
