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NJBMS - Volume 8, Issue 3, January - March 2018

Pages: 153-159
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The Study of P53immunoexpression In Carcinoma Cervix

Author: Dr.P.Aruna, Dr. L. Lakshmana rao

Category: Pathology


Background : Worldwide cervical carcinoma is responsible for about 5% of all cancer deaths in women. Loss of p53functions is believed to play an important role in the pathogenesis of carcinomas of the uterine cervix. Mutation of the p53 tumor suppressor gene is the most frequent genetic event described thus far in human cancer. Normal p53 protein has a very short half life and thus the protein level is too low to be identified immunohistochemically. In contrast most mutant p53 proteins have a longer half life and are easily detected by immunohistochemical methods.

Aim: The aim of the present study is to examine the over expression of p53 protein in different tumor types of carcinoma cervix.

Materials and methods:This is a retrospective study carried out in the 285 paraffin blocks from the cases of carcinoma cervix in the department of Pathology, Rajah Muthiah Medical College, Chidambaram. Immunohistochemical study using Dextran polymer conjugate-Two step visualization system is used besides H&E staining.

Results: Out of 285 cervical carcinoma biopsies 50 cases were randomly selected and subjected to immunohistochemical analysis for p53 protein.

p53 expression was found to be positive in 42 cases(84%),which include:

 39 squamous cell carcinoma

 1 adenocarcinoma

 I adenosquamous carcinoma

 1 small cell carcinoma

Conclusion: Loss of p53 function is having a key role in squamous cell carcinoma of cervix.

Keywords: Carcinoma cervix, p53 protein, immunohistochemistry.

DOI: 10.31975/NJBMS.2018.8306