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<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2d1 20170631//EN" "JATS-journalpublishing1.dtd">
      <Volume-Issue>Volume 4, Issue 1</Volume-Issue>
      <Season>July - September 2013</Season>
          <FirstName>RIJIED THOMPSON</FirstName>
          <FirstName>MARY HYDRINA</FirstName>
      <Abstract>Background and Objectives: Chromosomal aberrations, gene defects and environmental interactions between them are known to cause 20% to 40% of birth defects. Genome instability in the form of chromosomal breaks, deletions and translocations has been observed in the children with these anomalies. This study was attempted on non syndromic congenital skeletal malformations to correlate the phenotype with the extent of DNA damage. Materials and Methods: A total of 20 children were studied. 10 of them, from newborns to 5 years of age presenting with various skeletal malformations not attributed to any syndrome, in the absence of other system anomalies, formed the case material. A proportionate number of them matched for age and sex formed the controls. Peripheral lymphocytes from both, cases and controls were subjected to the standard Comet Assay. This very sensitive assay to measure DNA damage is a single cell gel electrophoretic technique where damaged DNA moves out of the cell towards the anode forming a comet, the length of the comet tail being a measure of DNA damage. Results: The skeletal deformities observed were Congenital Dysplasia Hip, Congenital Talipes Equino Varus and Arthrogryposis Multiplex Congenita. The mean tail length in cases  was 21.55 ìm and in controls was 1.992 ìm with very high statistical correlation (P value</Abstract>
      <Keywords>DNA Damage, Congenital Skeletal Malformations</Keywords>
        <Abstract>https://njbms.in/ubijournal-v1copy/journals/abstract.php?article_id=1545&amp;title=ESTIMATION OF DNA DAMAGE IN NON SYNDROMIC CONGENITAL SKELETAL MALFORMATIONS USING COMET ASSAY</Abstract>