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  <Article>
    <Journal>
      <PublisherName>njbms</PublisherName>
      <JournalTitle>NJBMS</JournalTitle>
      <PISSN>0976-6626</PISSN>
      <EISSN>2455-1740</EISSN>
      <Volume-Issue>Volume 3, Issue 1</Volume-Issue>
      <PartNumber/>
      <IssueTopic>Multidisciplinary</IssueTopic>
      <IssueLanguage>English</IssueLanguage>
      <Season>July - September 2012</Season>
      <SpecialIssue>N</SpecialIssue>
      <SupplementaryIssue>N</SupplementaryIssue>
      <IssueOA>Y</IssueOA>
      <PubDate>
        <Year>-0001</Year>
        <Month>11</Month>
        <Day>30</Day>
      </PubDate>
      <ArticleType>Microbiology</ArticleType>
      <ArticleTitle>COMPARATIVE EVALUATION OF CONVENTIONAL METHODS AND NESTED POLYMERASE CHAIN REACTION IN THE DIAGNOSIS OF EXTRA-PULMONARY TUBERCULOSIS</ArticleTitle>
      <SubTitle/>
      <ArticleLanguage>English</ArticleLanguage>
      <ArticleOA>Y</ArticleOA>
      <FirstPage>61</FirstPage>
      <LastPage>64</LastPage>
      <AuthorList>
        <Author>
          <FirstName>GILL</FirstName>
          <LastName>MANMEET</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>N</CorrespondingAuthor>
          <ORCID/>
          <FirstName>SHARMA</FirstName>
          <LastName>SARABJEET</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName>AGGARWAL</FirstName>
          <LastName>ARUNA</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName>KUKREJA</FirstName>
          <LastName>SAHIBA</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
        </Author>
      </AuthorList>
      <DOI/>
      <Abstract>Background: The conventional techniques like smear microscopy and culture suffer from low sensitivity for diagnosis of extra-pulmonary TB (EPTB). Polymerase Chain Reaction (PCR) is presently seen as a promising alternative to conventional techniques. This study evaluates IS6110 sequence based nested PCR (nPCR) for the detection of Mycobacterium tuberculosis (MTB) DNA directly from clinical samples of extra-pulmonary origin.Material and Methods: A total of 120 extra-pulmonary samples from the patients with history suggestive of tuberculosis were taken. All the samples were processed for Ziehl-Neelsan staining for acid fast bacilli (AFB), culture and PCR with primers targeting 123bp fragment of IS6110 of MTB complex.Results: A significant difference was seen in the sensitivities of conventional methods and PCR (p</Abstract>
      <AbstractLanguage>English</AbstractLanguage>
      <Keywords>Extra- pulmonary tuberculosis, Mycobacterium tuberculosis, nested polymerase chain reaction, IS6110 sequence.</Keywords>
      <URLs>
        <Abstract>https://njbms.in/ubijournal-v1copy/journals/abstract.php?article_id=1608&amp;title=COMPARATIVE EVALUATION OF CONVENTIONAL METHODS AND NESTED POLYMERASE CHAIN REACTION IN THE DIAGNOSIS OF EXTRA-PULMONARY TUBERCULOSIS</Abstract>
      </URLs>
      <References>
        <ReferencesarticleTitle>References</ReferencesarticleTitle>
        <ReferencesfirstPage>16</ReferencesfirstPage>
        <ReferenceslastPage>19</ReferenceslastPage>
        <References/>
      </References>
    </Journal>
  </Article>
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