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    <Journal>
      <PublisherName>njbms</PublisherName>
      <JournalTitle>NJBMS</JournalTitle>
      <PISSN>0976-6626</PISSN>
      <EISSN>2455-1740</EISSN>
      <Volume-Issue>Volume 7, Issue 2</Volume-Issue>
      <PartNumber/>
      <IssueTopic>Multidisciplinary</IssueTopic>
      <IssueLanguage>English</IssueLanguage>
      <Season>October - December 2016</Season>
      <SpecialIssue>N</SpecialIssue>
      <SupplementaryIssue>N</SupplementaryIssue>
      <IssueOA>Y</IssueOA>
      <PubDate>
        <Year>-0001</Year>
        <Month>11</Month>
        <Day>30</Day>
      </PubDate>
      <ArticleType>Microbiology</ArticleType>
      <ArticleTitle>Comparison Of Phenotypic And Genotypic Methods For The Detection Of Imipenem Resistant Enterobacteriaceae Isolates</ArticleTitle>
      <SubTitle/>
      <ArticleLanguage>English</ArticleLanguage>
      <ArticleOA>Y</ArticleOA>
      <FirstPage>106</FirstPage>
      <LastPage>112</LastPage>
      <AuthorList>
        <Author>
          <FirstName>C.Anuradha</FirstName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>N</CorrespondingAuthor>
          <ORCID/>
          <FirstName>S</FirstName>
          <LastName>.Mathavi</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName>R.Indra</FirstName>
          <LastName>Priyadharsini</LastName>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
          <FirstName/>
          <AuthorLanguage>English</AuthorLanguage>
          <Affiliation/>
          <CorrespondingAuthor>Y</CorrespondingAuthor>
          <ORCID/>
        </Author>
      </AuthorList>
      <DOI/>
      <Abstract>INTRODUCTION : The rapid spread of metallobetalactamases (MBL) is a diagnostic challenge in clinical microbiology as there are concerns regarding the reliability of the various phenotypic detection methods.AIMS AND OBJECTIVES : To assess the efficiency of the phenotypic assays namely the combined disc test (CDT), double disc synergy test (DDST) and the MBL E test as compared to PCR for the detection of MBLs in enterobacteriaceae.MATERIALS AND METHODS: Two hundred and four non-repetitive enterobacteriaceae isolates from clinical samples from October 2013 to September 2014 were included in the study.Isolates that showed a resistant zone ( &le; 19 mm ) to imipenem were screened by means of combined  disc   test   (CDT),  double  disc   synergy  test     (DDST)  and   E      test (imipenem/imipenem+EDTA  E strips, Biomeriuex). Multiplex PCR was done to confirm the presence of MBLgenes.RESULTS : The study showed 49 isolates resistant to imipenem, of which 12 ( 24.48% ) were positive for MBL production by both combined and double disc methods; 37 ( 75.52% ) showed positivity by combined disc only and 12 ( 24.48% )isolates by double disc method only.Twelve isolates showed a MIC of &ge;4&micro;g/mLby E test .PCR detected genes in 11 isolates(22.4%) only.CONCLUSION : Since phenotypic methods give variable results, it is not advisable to employ a single screening criterion for carbapenemase detection.Phenotypic methods though specific, do not differentiate between chromosomal and plasmid encoded genes and hence genotypic characterization ( PCR ) should be considered.</Abstract>
      <AbstractLanguage>English</AbstractLanguage>
      <Keywords>Phenotypic methods,genotypic methods,Imipenem,Enterobacteriaceae</Keywords>
      <URLs>
        <Abstract>https://njbms.in/ubijournal-v1copy/journals/abstract.php?article_id=1969&amp;title=Comparison Of Phenotypic And Genotypic Methods For The Detection Of Imipenem  Resistant Enterobacteriaceae Isolates</Abstract>
      </URLs>
      <References>
        <ReferencesarticleTitle>References</ReferencesarticleTitle>
        <ReferencesfirstPage>16</ReferencesfirstPage>
        <ReferenceslastPage>19</ReferenceslastPage>
        <References/>
      </References>
    </Journal>
  </Article>
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